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Rat brain-derived antisera prepared against human cytomegalovirus (HCMV) glycoproteins gB, gH, and gM, were used to determine the binding specificity of these glycoproteins to rat cell-surface receptors. A competitive inhibition radioimmunoassay (RICIA) was used to detect binding of viral glycoproteins to membranes of HEp-2 human tracheal carcinoma cells, Hela human cervical adenocarcinoma cells, and CV-1 mouse fibroblast cells. Our results suggest that the membranes of these cell lines possess receptors for HCMV glycoproteins gB, gH, and gM. Specific antibody in the antisera completely inhibited binding of HCMV glycoprotein to the receptors of the two Hela cell lines, whereas binding of gB and gH to HEp-2 cell receptors was inhibited by only 50 to 60%. The binding of HCMV glycoproteins to CV-1 cell receptors was not inhibited by antibody prepared against the gB, gH, or gM glycoproteins.Q:
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Uncaught (in promise) TypeError: _this.deployer.listen is not a function
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